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KMID : 0613820070170010120
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Journal of Life Science
2007 Volume.17 No. 1 p.120 ~ p.124
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Optimization of gibberellin production by Fusarium prolifertum KGL0401 and its involvement in waito-c rice growth
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Rim Soon-Ok
Lee Jin-Hyung
Lee Jin-Hyung
Rhee In-Koo
Kim Jong-Guk
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Abstract
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Fusarium proliferatum KGL0401 was previously isolated from Physalis alkekengi var. francheti plant roots and exhibited higher GA productivity than wild type Gibberella fujikuroi. The aim of this work was to find out an optimal culture condition for GA production. Various carbon(fructose, glucose, lactose, maltose, sucrose) and nitrogen(KNO₃, urea, glycine, NaNO₃, NH₄Cl) sources were used for this study. GAs activities were analysed by gas chromatography and mass spectrometry(GC-MS). The highest yield of GA₃ was found in the growth medium supplemented with sucrose as carbon source and NH₄Cl as nitrogen source. The optimum carbon-nitrogen concentration for GA₃ production was found to be 0.5 M:0.17 M. Supernatant was prepared from the culture fluid of F. proliferatum KGL0401 cultured for 7 days at 3 0℃ and the 10 ㎕ supernatant was treated with 2 leaf-rice seedling.
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KEYWORD
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Fusarium prolifertum KGL0401, Gibberella fujikuroi, Gibberellin, GC-MS analysis, waito-c
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